The objective of the current research was to make a character description of simple sequence repeats (SSR) derived from expressed sequence TAGs (EST) markers of dromedary camels (EST-SSR markers) and to conduct a practical analysis of these sequences for their application in comparative genomics and molecular genetics studies. A complete of 862 SSRs were discovered from 17155 EST sequences using the SSR Loca-tor software. 827 EST out of 17155 EST had SSRs, that 794 (96%), 31 (3. 8%) and 2 (0. 2%) of them con-tained 1, 2 and 3 SSRs, respectively. The dimeric motifs were the most abundant SSRs (38. 86%), followed by 27. 15%, 21. 46%, 6. 96%, and 5. 57% for tri-, hexa-, tetra-and pentameric motifs. The most plentiful dimer, trimer, tetramer, pentamer and hexamer motif were AC/TG (54%), GCC/GGC (19. 2%), TTTA (13. 3%), AAAAG (10. 4%) and AACCAC (67. 6 %), respectively. BLASTX was used to examine the final non-redundant EST-SSRs. Almost all of EST-SSRs were found out to be protected in the macromolecule catabolic process and RNA processing and splicing. EST-SSR markers might be applied as a novel resource of useful markers in the biological survey. Also, these markers may be a valuable source for further molecular genetics and genomics research of camels and related species.